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Transmisi Transovarial Virus Dengue pada Telur Nyamuk Aedes aegypti (L.)

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The ability of dengue virus to maintain its existence in nature through two mechanisms, both horizontal and vertical transmission (transovarial) of the infective female mosquitoes to the next generation. This study aims to investigate the transovarial transmission and transovarial infection rate (TIR) of dengue virus in eggs Aedes aegypti infected mother has a peroral virus DEN-2. This study is an experimental study in the laboratory. The population of the study was Ae. aegypti adults who have previously been infected with DEN-2 virus orally and proved to be infected with DEN-2 transovarially (Fl). The research sample was egg of Ae. aegypti from F2 generation which colonized from DEN-2 transovarially infected Ae. aegypti (Fl). Egg squash preparations made as many as 50 samples from jive difJerent mosquito parents. The presence of dengue virus antigen in mosquitoes FO and Fl were checked by SPBC immunocytochemistry method and using monoclonal antibodies DSSC7 (l: 50) as standardized primary antibodies. The results shows the existence of transovarial transmission of dengue virus in eggs Ae. aegypti (F2) were seen in squash preparations in the form of a brownish color egg spread on embryonic tissues (TIR= 52%). It concludes that dengue virus is able to be transmitted vertically through the egg.

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Detail Information

Series Title

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Call Number

Aspirator, 4 (2) 2012: 53-58

Publisher

Jawa Barat : Loka Litbangkes P2B2 Ciamis., 2012

Collation

6p.

Language

ISBN/ISSN

2085-4102

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

DENGUE VIRUS

Specific Detail Info

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