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Systematic Review Reprogramming Yang Relatif Aman Dengan Protein Rekombinan Non-Onkogenik Sebagai Sumber Sel Punca

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Background: Stem cell reprogramming has been used as a source of pluripotent cells due to controversy associated with utilization of embryonic stem cells. However, some of reprogramming methods used involve risky genetic modifications. The objective of this review is to discuss a safer reprogramming method using non-oncogenic recombinant proteins.
Methods: This article was made with prior search of references using search engine Google, NeB! and PubMed with keywords, such as reprogramming, recombinant protein, iPS (induced pluripotent stem cells), and ancogene. Through systematic sorting, the chosen references include 7 reviews, 18 experimental study reports, 2 ebooks and 2 webpages.
Results: Several studies of reprogramming using recombinant proteins were reported producing three to jive colonies of iPS around day 30 and can be expanded up to more than 30 passages. Small molecule such as valproic acid increases its efficiency (3 colonies per 5x104 cell), compared to that without chemical treatments (5 colonies per 5x105 cel!). Utilization of oncogenic factors, e.g., c-Myc, Klf-4 in the process has been strongly associated with tumor formations. LIN-28 could pramote oncogenicity, yet it does not set off cell hyperprolijeration straightforwardly.
Conclusion: Reprogrnmming using recombinant protein generates iPS without modifYing target cell genome despite its low efficiency and involvement of oncogenic factors.
Keywords: induced pluripotent stem cell (iPS), reprogramming, recombinant protein, oncogene

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Jurnal Biotek Medisiana Indonesia, 1(1) 2012 : 25-

Penerbit

Jakarta : Pusat Biomedis dan Teknologi Dasar Kesehatan., 2012

Deskripsi Fisik

6p

Bahasa

ISBN/ISSN

2301-5810

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

STEM CELL

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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