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Genetic variability of Candida albicans in HIV/AIDS patient with and without ARV therapy and non-HIV/AIDS

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Background: Oral candidiasis is the mostlyfound oral manifestauon in HIV/AIDS infected patient caused by immunocompromised especially immunodeficiency. Clinical symptoms is severe pain in oral cavity and dry mouth because of xerostomia which cause the loss of appetite. Candida albicans (C albicans) is normal flora in oral cavity which plays as opportunistic pathogen and also the cause of oral candidiasis. Almost 90% of HIV-infected patient have oral candidiasis. This condition is clinical problem whicli has not been well-managed yet. C albicans colonized oral mucous cavity has different genetic variability for each strain. Phenotype of C albicans has been determined by genetic factor and environtment. This condition stimulate differences of genotype among various strain of C albicans in the world. Purpose: The purpose of this research is to analyze the genetic variability of C albicans which colonized in che mucous oral cavity of HIV/AIDS patient in Surabaya in the treatment with. and without ARV therapy and non-HIV/AIDS.
Methods: This research has been identify and characterize the prevalent strain of C. albicans isolat in Surabaya (East Java) in HIV/AIDS infected patient with oral candidiasis by method of Iatron candidal check. The highlight of this research including cytology examination by Papanicoloau staining, C albicans culture, spheroplast making, DNA isolation and genetic variability checking by randomly amplyfied polymorphism DNA (RA PD).
Results: C albicans colonizing oral mucosa of non-HIV patients had a predisposition of farther genetic relationship (genetic distance of 0.452) with C albicans colonizing oral mucosa of HIV ARV and HIV non-ARV patients. The genetic distance was ranging between 0 and 1, where 9 was long genetic distance and 1 was short genetic distance. In contrast, C albicanscoloniiing oral mucosa of HIV ARV have predisposition of closer genetic relationship (genetic distance of 0.762) with C albicans colonizing oral mucosa of HIV non-ARV patients.
Conclusion: The conclusion of this research were C albicans colonizing HIV/AIDS patiens with and without ARV showed no high genetic variability between C albicans isolate in HIV patients. Therefore, the character of C albicans colonizing HJV ARV and HIV non-ARV patients had similar genotype predisposition of closer relationship value with C albicans colonizing oral mucosa non-HIV patients.
Key words: Candida albicans, HIV/AIDS, oral candidosis, RAPD

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Dental Journal, 45 (1) 2012 : 28-34

Publisher

Surabaya : FKG Universitas Airlangga., 2012

Collation

7p

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ISBN/ISSN

1978-3728

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Subject(s)

HIV
AQUAIRED IMMUNODEFICIENCY SYNDROME

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