Proteksi Probiotik pada Mukosa Ileum Mencit yang Terpajan Lipopolisakarida Escherichia Coli | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Proteksi Probiotik pada Mukosa Ileum Mencit yang Terpajan Lipopolisakarida Escherichia Coli

Alpha Fardah Athiyyah - Personal Name; Andy Darma - Personal Name; Ariani Setiawati - Personal Name; Anang Endaryanto - Personal Name; I Ketut Sudiana - Personal Name; Reza Ranuh - Personal Name; Subijanto MS - Personal Name;

Background: Gastrointestinal infection is one of the major causes of morbidity in children. Diarrhea is the common manifestation. Diarrheal prevention needs a balance mucosal immune system. Probiotic used in prevention of gastrointestinal infection needs to be considered. The aim of the study is to prove probiotic protection in ileal mucosa after lipopolysaccaride (LPS) Escherichia coli 055:B5 inoculation anatomically and immunologically.
Method: Experimental study with male Balb/c, age 10-12 weeks, body weight 30-40g and randomized into treatment group and placebo group. Treatment group received mix probiotic for 14 days and on day 15 were inoculated by LPS Escherichia coli 055:B5, and day 16-21 were given mix probiotic again. Placebo group received LPS Escherichia coli 055:B5 on day 15. Mice necropsy were conducted at day 22. 1mmunohistochemistry examination used to lookfor amount expression cellfor IL-2, IFN-γ, TNF-α, IL-4, IL-5, IL-6, IL-10 dan TGF-β in ileal mucosa. Scanning electron microscope was used to see ileal mucosal structure. Statistic analysis used in this study was multivariate analysis.
Results: Sixteen Balblc mices were randomized into 2 groups, each group consist 8 mices. There was significant difference on amount of cells expression 1L-5 (p=0.022), 1L-6 (p=0.05), and also on amount of cells expression TGF-β (p=0.036). On probiotic-LPS group, there was no Th1 domination but on the other hand, Treg became dominant. Th1 and Th2 response were still balance.
Structural damages occurred in LPS group and did not occur in probiotic-LPS group.
Conclusion: Probiotic protection in ileal Balblc mice mucosa after LPS Escherichia coli 055:B5 inoculation is through anatomy and immunology.changes.
Keywords: Probiotic, LPS, Escherichia coli, protection, immunology

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Location name is not set Media Medika Indonesiana, 46 (2) 2012 : 80-85

A0003375

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Series Title: -
Call Number: Media Medika Indonesiana, 46 (2) 2012 : 80-85
Publisher: Semarang : FK Diponegoro Semarang., 2012
Collation: 6p
Language
ISBN/ISSN: 0126-1762
Classification: -
Content Type: -
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Carrier Type: -
Edition: -
Subject(s): escherichia coli
LIPOPOLYSACCHARIDES
PROBIOTICS
Specific Detail Info: -
Statement of Responsibility: -

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