Effect of temperature and storage duration of Aedes aegypti mosquito specimens artificially infected with dengue-3 virus on the results of immunohistochemical examination | Perpustakaan dan Galeri Kebijakan Kesehatan

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Effect of temperature and storage duration of Aedes aegypti mosquito specimens artificially infected with dengue-3 virus on the results of immunohistochemical examination

Susilawati - Nama Orang; Sitti Rohmah Umniyati - Nama Orang; Tri Baskoro Tunggul Satoto - Nama Orang;

To confirm the presence of any dengue viral in a mosquito, mosquito's head can be squashed on a slide and stained with immunohistochemical staining. The remaining samples then can be stored in the cryo freezer at -80oC to avoid specimen damage. However, for laboratories with limited facilities, with only a refrigerator with a temperature range of -20oC to 4°C is available, examination to evaluate whether the dengue antigen can still be detected in specimens stored at these temperature is necessary. It was a quasi-experimental study. Three to five-day-old adult female Aedes aegypti mosquitoes were injected intrathoracically with 'dengue-3 (OENV-3) strain H-87 virus and then maintained for about 7 days. The dengue viral antigen on mosquitoes was identified using immunohistochemical method after stored at a temperature of 4°C, -20oC, -80oC for 2, 4 and 8 weeks. Mosquito specimens that were not stored were used as a positive control. Kappa value was counted to analyze level of agreements between two observers. Two-way Anova was used to analyze mean positive rates. Kappa value showed poor agreement (0.00- 0.16) between two observers when the specimens were stored at 4°C for 2-8 weeks, and showed good agreement (Kappa value of 0.77), when stored at -20oC for 4 weeks. The kappa value show ed very good agreement (0.90-0.92) when the specimens were stored at -20oC for 2 weeks, and at -800e for 2-8 weeks. Mean positive rates of the specimens stored at 4°C were significantly lower (p0.05). In conclusion, availability of the dengue viral antigen on mosquito specimens was influenced by temperature and storage duration of the specimens.

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Belum memasukkan lokasi Journal of The Medical Sciences, 44 (1) 2012 : 35-

A0003274

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Informasi Detail

Judul Seri: -
No. Panggil: Journal of The Medical Sciences, 44 (1) 2012 : 35-
Penerbit: Yoyakarta : FK Universitas Gajah Mada Yogyakarta., 2012
Deskripsi Fisik: 6p
Bahasa
ISBN/ISSN: 0126-1312
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: aedes
DENGUE VIRUS
IMMUNOHISTOCHEMISTRY
Info Detail Spesifik: -
Pernyataan Tanggungjawab: -

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