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Effect of temperature and storage duration of Aedes aegypti mosquito specimens artificially infected with dengue-3 virus on the results of immunohistochemical examination

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To confirm the presence of any dengue viral in a mosquito, mosquito's head can be squashed on a slide and stained with immunohistochemical staining. The remaining samples then can be stored in the cryo freezer at -80oC to avoid specimen damage. However, for laboratories with limited facilities, with only a refrigerator with a temperature range of -20oC to 4°C is available, examination to evaluate whether the dengue antigen can still be detected in specimens stored at these temperature is necessary. It was a quasi-experimental study. Three to five-day-old adult female Aedes aegypti mosquitoes were injected intrathoracically with 'dengue-3 (OENV-3) strain H-87 virus and then maintained for about 7 days. The dengue viral antigen on mosquitoes was identified using immunohistochemical method after stored at a temperature of 4°C, -20oC, -80oC for 2, 4 and 8 weeks. Mosquito specimens that were not stored were used as a positive control. Kappa value was counted to analyze level of agreements between two observers. Two-way Anova was used to analyze mean positive rates. Kappa value showed poor agreement (0.00- 0.16) between two observers when the specimens were stored at 4°C for 2-8 weeks, and showed good agreement (Kappa value of 0.77), when stored at -20oC for 4 weeks. The kappa value show ed very good agreement (0.90-0.92) when the specimens were stored at -20oC for 2 weeks, and at -800e for 2-8 weeks. Mean positive rates of the specimens stored at 4°C were significantly lower (p0.05). In conclusion, availability of the dengue viral antigen on mosquito specimens was influenced by temperature and storage duration of the specimens.

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Detail Information

Series Title

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Call Number

Journal of The Medical Sciences, 44 (1) 2012 : 35-

Publisher

Yoyakarta : FK Universitas Gajah Mada Yogyakarta., 2012

Collation

6p

Language

ISBN/ISSN

0126-1312

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Media Type

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Carrier Type

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Edition

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Subject(s)

aedes
DENGUE VIRUS
IMMUNOHISTOCHEMISTRY

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