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Serum testosterone level and active caspase-3 of Leydig cells of diabetic Sprague.-Dawley male rats after administration of soybean (Glycin max)powder suspension

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Diabetes mellitus (DM) is known as the main death cau se in the world. This disease cau ses acute and chronic complication. The comm on chronic complication on male reproduction system is the decrease of testosterone level. This hormone is produced in the Leydig cells. Soybeans (Glycin max) has been used in the management of DM to maintain blood glucose level. However, the effect of soybean on serum testosterone level is still unclear. The aim of this study was to evaluate serum testosterone level and caspase-3 active of Leydig cells of diabetic Sprague-Dawley (SD) male rats model induced by streptozotocine (STZ) after oral ingestion of soybean powder suspension. This was an experimental study with pre and post test control group design. Thirty SD male rats, aged 11-12 weeks with body weight (BW) of 200-250 g were divided into 5 groups with 6 rats in each group. The first group (G 1) was normal rat control and the second group (G2) was diabetic rat control. The third to fifth group (G3, G4 and G5) were diabetic rat treatment with oral ingestion of soybean powder suspension that was given once a day during 4 weeks with dose of 400; 800 and 1600 mg/kg BW, respectively. The serum testosterone level was measured by an ELlSA and active caspase-3 of Leydig cells were measured by an immunohistochemistry method. The result showed that the serum testosterone level and active casadse-S Leydig cells of diabetic rats model induced by STZ were not significantly different after oral ingestion of soybean powder suspension with dose of 400, 800 and 1600 mg/kg BW (p > 0.05). In conclusion, soybean powder suspension did not affect the serum testosterone level and active caspase-3 Leydig cell in diabetic rats.

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Series Title

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Call Number

Journal of The Medical Sciences, 44 (1) 2012 : 18-

Publisher

Yogyakarta : Bagian Ilmu Kesehatan Anak FK UNPAD \r RSHS., 2012

Collation

13p

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ISBN/ISSN

0126-1312

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Edition

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Subject(s)

soybeans
testosterone

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