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Activity of α-terpineol as a potential anticancer candidate: cytotoxicity, proapoptotic and antiproliferative evaluation in TD47 cell lines

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Previous study has shown th at α-terpineol has a cytotoxic effect on NCI-H69 cancer cell line. This study aimed to evaluate cytotoxicity, proapoptotic, and antiproliferative activities of α -terpineol in T470 celllines. The cytotoxic activity of α-terpineol was determined using MTT cell viability assay, while apoptosis was tested by the acridine orange-ethidium bromide staining method. The cell cycle was evaluated by flowcytometry method. The results showed that α-terpineol had cytotoxic effect on the T470 cells with the IC50 value of 135.00 ± 8.74 µM. Furthermore, α-terpineol induced the cells apoptosis in a dose-dependent manner. Flowcytometry analysis showed that α-terpineol induced the T470 cell distribution in Go/G1 phase, however inhibited cell distribution in S phase. This study suggests th at α-terpineol has a potential anticancer activity.
ABSTRAK
Penelitian sebelumnya menunjukkan bahwa a-terpineol mempunyai efek sitotoksik pada sel kanker NCI-H69. Penelitian ini bertujuan mengkaji aktivitas sitotoksisitas, proapoptotik dan antiproliferatif dari alpfa terpinol in sel kanker T 470. Aktivitas sitotoksisitas α-terpineol ditentukan dengan MTT cell viability assay, sedangkan apoptosis diuji dengan metode pewarnaan akridin oranye-etidium bromida. Siklus sel dievaluasi dengan metode flowsitometri. Hasil penelitian menunjukkan α-terpineol mempunyai efek sitotokik pada sel T470 dengan nilai IC50 135,00 ± 8.74 µM. Selain itu α-terpineol menginduksi apoptosis yang bergantung konsentrasi. Analisis flowsitometri menunjukkan α-terpineol menginduksi distribusi sel T 470 pada fase Go/G1 namun demikian snqharnbat distribusi sel pada fase S. Penelitian ini menunjukkan bahwa α-terpineol mempunyai aktivitas anti kanker potensial.
Keywords: α-terpineol - T 470 cell line - cytotoxicity - proapoptotic - antiproliferative

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Series Title

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Call Number

Journal of The Medical Sciences, 44 (1) 2012 : 10-

Publisher

Yogyakarta : Bagian Ilmu Kesehatan Anak FK UNPAD \r RSHS., 2012

Collation

8p

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ISBN/ISSN

0126-1312

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Edition

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Subject(s)

anticarcinogenic agents
CYTOTOXINS

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