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Correlation between hypoxia inducible factor -1α and renin expression in rats kidney induced by cobaIt chloride

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Background: Cobalt chloride can be used as an agent to stabilize hypoxia inducible factor-1α (HIF-1α) and to imitate hypoxia without low levels of oxygen inside the body. We intended to Investigate if there was any regulation of renin expression by HIF -1α. Therefore, we conducted several studies to elarify this possibility starting with the induction of hypoxic mimicry in rats by intra-peritoneal (IP) injection of cobait chloride (CoCI2) to obtain the levels and pattem of HIF-1α and renin mRNA and protein expression.
Methods: Twenty-four rats were randomly divided into four groups, control group and Incubation groups 2,8, and 24 hours after intra-peritoneal injection of 30 mg CoCl2 per kg BW. After the rats were sacrificed, kidneys were excised, weighed and kidney weight compared to BW. Tissue parameters were measured such as RNA concentration, HIF-1α protein by ELISA, and renin mRNA by RT-PCR.
Results: Differences between the groups in the ratios ofkidney weight to BW and in the concentrations of HIF-1α protein were statistically not significant (p > 0.05). Relative expression of renin mRNA increased markedly starting 8 hours after CoCI2 IP injection (30 times over controis) and further rising until 24 hours (2465 times over controis). Correlation between HIF-1α and renin mRNA by Pearson analysis was strongly positive, but not significant (R = 0.91; P = 0.09). Conclusion: Renin gene regulation in renal hypoxic mimicry strongly correlates with HIF-1α.
(Keywords: Cobalt chloride (Co CI2), hypoxia inducible factor-1α (HIF-1α), renin

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Series Title

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Call Number

Medical Journal Indonesia, 21 (3) 2012 : 128-132

Publisher

Jakarta : FK UI., 2012

Collation

5p

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ISBN/ISSN

2252-8083

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Subject(s)

COBALT
ANOXIA

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