Efek Ekstrak Etanolik Daun Sirsak pada Proliferasi dan Apoptosis Sel Hela yang Dimediasi oleh p53 (The Effect of Annona Muricata Leaf on Proliferation and Appoptosis of HeLa Cells Mediated by p53) | Perpustakaan dan Galeri Kebijakan Kesehatan

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Efek Ekstrak Etanolik Daun Sirsak pada Proliferasi dan Apoptosis Sel Hela yang Dimediasi oleh p53 (The Effect of Annona Muricata Leaf on Proliferation and Appoptosis of HeLa Cells Mediated by p53)

Ermin Rachmawati - Nama Orang; Setya wati Karyono - Nama Orang; Hidayat Suyuti - Nama Orang;

Cervical cancer is the second leading cause of cancer mortality in women worldwide, causing 240.000 deaths annually. Annona muricata leaves extract have emerged as potentially promising anti cancer drugs. The purpose of this study was to investigate the effect of ethanolic extract of soursoup (Annona muricata) leaves on inhibiting cervical cancer growth and induce apoptosis which is mediated by p53 stabilization and activation. An in vitro experiment was performed using HeLa cell culture. The proliferation inhibition was measured by MTT assay. Apoptosis was detected by flowcytometry using Annexin V Biotin Apoptosis Oetection Kit. Confirmation and calculation of p53 expression was determined by imunocytochemistry. Ethanolic extract of Annona muricata leaves were effectively inhibit HeLa cells proliferation dase dependent manner by48 hours exposure. The IC5o values ofethanolic extract of Annona muricata leaves was 111. 75 µg/ml. The 200 µg/ml extract induced apoptosis in HeLa cells time dependent manner. The extract also increased the expression of p53 in HeLa cells dose (25, 50, 100, 200µg/ml) and time (24 and 48 hour) dependent manner. It can be concluded that ethanolic extract of soursoup leaves inhibit Helii cell growth and induced apoptosis in HeLa cells. The mechanism of extract- inhibiting proliferation and inducing apoptosis might be associated with the stabilization and activation of p53.
Keywords: Annona muricata leaves, apoptosis, ethanolic extroct, proliferation, p53 expression

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Belum memasukkan lokasi Jurnal Kedokteran Brawijaya, 27 (1) 2012 : 28-33

A0003102

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Judul Seri: -
No. Panggil: Jurnal Kedokteran Brawijaya, 27 (1) 2012 : 28-33
Penerbit: Mataram : FK Universitas Brawijaya., 2012
Deskripsi Fisik: 6p
Bahasa
ISBN/ISSN: 0216-9347
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