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Alpa-Lipoic Acid, Inhibit the Decrease of Collagen Deposition in Ultraviolet B-irradiated Cultured Normal Human Skin Fibroblasts Cell Culture

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Repeated ultraviolet B (UVB) irradiation on human skin has been considered to be responsible in premature aging process because UVB has been proved to inhibit collagen deposition and accelerates collagen degradation. Clinical studies because UVB has been proved to inhibit collagen deposition and accelerates collagen degradation in UVB irradiated normal human skin fibroblasts culture has not been reported. The aim of the study was to investigates the affect of ALA on collagen deposition and degradation in UVB irradiated cultured normal human skinfibroblasts. Culture of normal human skinfibroblasts were treated with 0, 125,250,500 µM ALA diluted in complete Dulbecco’s Modified Eagle’s Medium(DMEM) and irradiated with 300 mj/cm2 UVB. The mean collagen diposition and degradation’s level were measured by Sirius red assay and read with spectrophotometer at ʎ 550 nm. Mean difference of collagen deposition as expressed by optical density (OD) between normal human skin fibroblasts cell after UVB irradiation and without UVB irradiation was analyzed by one by Wicoxon signed-ranks test and Friedman test and Fibroblasts cell after UVB irradiation was analyzed by Wilcoxon signed-ranks test and Friedman test, while mean difference collagen degradation was analyzed by one way analyzed-ranks test and friedman test, while mean difference collagen degradation was analyzed by one way analysis of variance (ANOVA) and paired t test with 95% confidence level (p0.05). In conclusion. ALA inhibited the decrease of collagen deposition, but did not inhibit collagen degradation in UVB irradiated normal human skin fibroblasts culture.

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Journal of the Medical Sciences:43(2)2011:94-98

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Yogyakarta : Faculty of Medicine Gadjah Mada Unviersity., 2011

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4p..

Language

English

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0126-1312

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