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Endothelial Cell Cultured on HA/TCP/Chitosan Scaffoid for Bone Tissue Engineering

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Angionesis is crucial for the sucess of bone recomstructionthrough tissue enginering. Currently, is still not known the activity of endothelial cells that is responsible for blood vessel formation, cultured in HA/TCP/chitoson scaffold. The ability of the scafflod to facilitate the proliferation and migration of endothelial cell to from blood vessel is essenitial for cell servival especially in the inner area of the scaffold that is susceptible for cell death of adequate vascularization is not occurred. The purpose of thie study was to evaluate the porosity of HA/TC/chitosan scaffoid and the biocompatibility of HA/TCP/chitosan scaffold to endothelial cells. Endothelial cell were isolated from umbilical vein (human umbilical vein endothelial cells/HUVC).HA/TCP chitosan scffold was made from two gelling agents and various basic washing solations. The characteristic of scaffold was examined by scannig electron microscopy. The activity of HUVEC was evaluated by MTT assay. Initial average scoffold porosity size range from 68µm and increased up to 134 µm after 7 days incubation with 10 mg/L. Lysazyme. There was no significant difference in the viability of HU/VEC incubated with the scaffold compared to control. HA/TCP/chiltosan has a good biocompatibility for HUVEC. This condition supports the activity of HUVEC in the scaffold for angiogenesis process, to provide osygen and nutrient necessary for asteoblast

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Detail Information

Series Title

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Call Number

Dental Journal, 44(2)2011:67-71

Publisher

Surabaya : Erlangga University Press., 2011

Collation

4p.

Language

English

ISBN/ISSN

1979-3728

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

bone and bones
CHITOSAN
ENDOTHELIAL CELL

Specific Detail Info

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