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Cytotoxicity Difference of 316L Stainless Steel and Titanium Reconstruction Plate

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Pare titanium is the most biocompatible material today and used as a gold standard for metalic. However stainess steel is still being used as implanty because of its strength, dutility, lower price, corrosion resistant and biocompatibility.Tis study was done to revealed the cytotoxicity difference between reconstruction plate made of 3161, steel and of commercially pare (CP)titanium in baby hanster kidney 21(BHK-211) fibroblast culture through MTT assay. Method: Eight samples were prepared from reconstruction plates made of stainless steel type 316L grade 2 (Coen’s reconstruction plate) that had been cut into cylindrical from of 2 mm in diameter and 3 mm long. The other one were male of CP titanium (STEMA Gmbh) of 2 mm in diameter and 2.2 mm long; and had been cleaned with slica paper and ultrasonic cleaner, and sterilized in autoclave at 121⁰C for 20 minutes . Both samples were bathed into microplate well containing 50 µl of fibroblast cells with 2x10pangkat 2 density in Rosewell Park Memoriat Institute-1640 (RPMI-1641) media, spinned an 30 rpn for 5 minutes. Microplate well was incubated for 24 and 45 hours in 37⁰ C. After 24 hours, each well that will be read at 24 hour were added with 50 µl solution containig 5mg/MTT reagent in phosphate buffer saline (PBS) solution. Then reincubated for 4 hours in CO₂ 10% and 37⁰C Colorometric assay with MTT was used to evaluate viability of the cells population after 24 hours. Then each well were added with 50 µl dimethyl sulfoxide (DMS)) and reincubated for 5 minutes in 37⁰C, the wells were read using ELISA reader in 620 nm wavelength. Same were done for the wells that will be read in 45 hours. Each data were tabulated and analyzed using independent T-test with significantce of 5%. Result This study showed that the percentage of living fibroblat after exposure to 316L stainless sted recon struction plate was 61.58% after 24 hours and 62.33% after 48 hours. And after exposure to titanium reconstruction plate, the percentage of living fibroblast was 98.69%after 24 hours and 82.24% after 48 hours. Based an cytotoxicity parameter (CD5%) both recontruction plate made of 316L stainless steel or titanium showed as a non-materials steel plate showed lower cytotxicity level compared to titanium. Therefore a reconstruction plate made from stainless steel type 316L can be used as a safe material for mandibular recontruction

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Dental Journal, 44(1)2011:7-11

Penerbit

Surabaya : Airlangga University Press, Badan Litbangkes., 2011

Deskripsi Fisik

4p.

Bahasa

English

ISBN/ISSN

1979-3728

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

CYTOTOXINS
TITANIUM

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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