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Pemeriksaan Real-time PCR dalam Diagnosis Pneumonia Pneumocystis
Pneumocystis penumonia (PCP) is an important opportunistic infection in HIV –infected patientsand other immune system disorders. Diagnosis of PCP remains a challenge becaus the clinical symptoms, routine laboratory and radiological examinations are not typical. The proven diagnosis must be performed by finding P. Jiroved in histopathologic preparations with conventional or immunocytochemical stained that require special clinical specimens, high laboratory skills and time consumed. The development of molecualr biology using polymerase chain reaction method (PCR) allows diagnosis of PCP from more easily obtained specimens, but can not distinguish the circumstances of commensal with pathogens Development of real time PCR allows the quantitative examination to distringuish that circumstances in less time.
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