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Pemeriksaan Real-time PCR dalam Diagnosis Pneumonia Pneumocystis
Pneumocystis pneumonia (PCP) is an important opportunistic infection in HIV-intracted patients and other immune system disorders. Diagnosis of PCP remains a challenge because the clinical symptoms, reutine laboratory and rediological examinations are not typical. The proven diagnosis must be performed by finding P. Jiroved in histopathologic preparations with conventional or immunocytochemical staned that require special clinical specimens, high laboratory skills and time consumed. The development of molecular biology using polymerase chain reaction method (PCR) allows diagnostic of PCP from more easily obtained specimens, but can not distinguish the cicumstances of commensal with parthogens. Development of real time PCR allows the quantitative examination to distinguish that circumstances in less time.
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