Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

Seruni K.U. Freisleben - Personal Name; Jenny Hidayat - Personal Name; Hans Joachim Freisleben - Personal Name;

Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage.

Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10 controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status were measured. Isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (EPR) spectroscopy using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA.

Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. Vitamin E, group C: 21.8 vs T: 6.2 μmol/L) and reactive thiols (C: 144 vs. T: 61 μmol/L) were considerably lower in transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 μmol/L) and of tissue cell damage, i.e., high transaminases plasma levels. Non-transfused thalassemia patients have slight signs of oxidative stress, but no significant indication of cell damage. Erythrocyte membrane parameters from EPR spectroscopy differ considerably between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized.

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Location name is not set Medical Journal of Indonesia:20(3)2011:178-184

A0002090

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Series Title: -
Call Number: Medical Journal of Indonesia:20(3)2011:178-184
Publisher: Jakarta : Universitas Indonesia., 2011
Collation: 6p.
Language: English
ISBN/ISSN: 0853-1773
Classification: -
Content Type: -
Media Type: -
Carrier Type: -
Edition: -
Subject(s): lipids
Plasma
OXIDATIVE STRESS
beta-thalassemia
Specific Detail Info: -
Statement of Responsibility: -

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