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Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi

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The culture media commonly used for isolation are imported and expensive. Many organic materials are naturally decomposed from complex organic compounds to simple ones by microbes. Based on this principles, this study wants to make isolation media with ripe coconut waste-water as based substance, so it can be considered as economical culture media. The method was laboratoric experimental by isolating tested bacteria with ripe coconut waste-water as based substance. The composition of the media were adjusted with MacConkey agar and blood agar base. Standard media were MacConkey agar and blood agar base. Control media were agar 15 g/L media and ripe coconut waste-water agar media. Tested bacteria were a number species of Enterobacteriaceae and positive gram cocci. The evaluated variables were macroscopic and microscopic images. Data was analized by Wilcoxon matched
pairs test and sign test methods. This study did not find a significant differences (p>0.05) between standard media and ripe coconut waste-water media. Specific characteristics of tested bacteria, such as red colonies, mucoid, and hemolitic zone, were similar between standard media and ripe coconut waste-water media. The conclusion is ripe
coconut waste-water can be used as base for isolation media substance of Enterobacteriaceae and gram positive
cocci. [MKB. 2011;43(3):117–21].

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Series Title

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Call Number

Majalah Kedokteran Bandung, 43 (3) 2011 : 117-121

Publisher

Bandung : FK. Universitas Padjadjaran., 2011

Collation

4p.

Language

ISBN/ISSN

0126-074X

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

COCOS
CULTURE MEDIA

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