Asam L Askorbat Meningkatkan Viabilitas HUVEC dalam Kultur P. falciparum yang Diinkubasi dengan Artemisinin Bergantung Konsentrasi | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Asam L Askorbat Meningkatkan Viabilitas HUVEC dalam Kultur P. falciparum yang Diinkubasi dengan Artemisinin Bergantung Konsentrasi

Yuwono - Personal Name; Imam Supardi - Personal Name; Sumarjati SA - Personal Name; Sadeli Masra - Personal Name;

Artemisinin via overproduction of free radical acts as a potent drug against multi-drug resistant Plasmodium. because of new permeation pathway evidence at parasitized red blood cell membrane, L ascorbic acid as hydrophilic antioxidant hopefully can’t penetrate the membrane so that it won’t interfere the artemisinin antimalarial effect rather it protects the host cell out of parasitized erythrocytes. The aim of this study was to determine its effect against human umbilical vein endothelial cell (HUVEC) viability, glutathione sulfa-hydryl (GSH), and malondialdehyde
(MDA) concentration in Plasmodium falciparum culture treated with artemisinin. This experiment was conducted at Eijkman Insitute for Molecular Biology, Jakarta from January 2007 to January 2008. Plasmodium falciparum 3D7 strain culture was incubated with IC50 of artemisinin and a wide concentration range of L ascorbic acid for
24 hours in a candle jar at 37oC incubator. GSH and MDA concentration were measured from the supernatant using spectrophotometer. HUVEC viability was measured in P. falciparum-HUVEC co-cultivation incubated with artemisinin IC50 and L ascorbic acid. The result was examined and analyzed using analysis of variance (ANOVA) and Tukey honestly significant difference (HSD)/Scheffe. It showed that 20 μM and 100 μM L ascorbic acid raised the HUVEC viability. It was concluded that L ascorbic acid concentration dependently increases HUVEC viability in P. falciparum culture incubated with artemisinin. [MKB.2011;43(2):66–71].

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Location name is not set Majalah Kedokteran Bandung:43(2)2011:60-65

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Call Number: Majalah Kedokteran Bandug: 43(2)2011:66-71
Publisher: Bandung : FK. Universitas Padjadjaran., 2011
Collation: 5p.
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ISBN/ISSN: 0126-074X
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