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Uji Sitotoksistas Tripen pentasiklik daun Eupatorium Inulifolium HBK terhasap Sel Mieloma dan Studi Dockingnya (Cytotoxicity test Pentacyclic Triterlenes of Epatorium Inulifolium HBK on Myoloma Cells Their Docking Study)

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A test of cytotoxic activity of pentacyclic triterpenes 12,13-Dihydro-1-α- amirin-20-30-en-3-acetate and 12,13-Dyhydro-α-amirin-20-30-en-30l compounds on, myeloma cells and their docking has been conducted. Two compounds were treated on myeloma cell which their cells densities have been determined. They were subssequently incubated with a series of compound dosage from 2000 µg/ml to 125 µg/ml. This research aims to determine the level in which both the compounds influence the myeloma cells MTTT method (reactor 3-(4,5- Dimetilitiazol-2-ll)-2,5-difenil tetrazollium bromide) and their docking that react to receptor 1XXX. Activity of compounds on myeloma cell after 24 hour incubation has shown that the values of IC50 for 12,13-dihydro-α- amirin-20,30-en-3=acetate and 12,13 –Dihydro-αDihydro-α-amirin-20,30l are 0.428 mM and 1.515 mM, respectively. In this research, doxorubicin is used as a positive control. The IC50 value of doxorubicin is 6.896 x10 µg/mL. Result show that docking score for 12,13-dihydro-α-amirin-2-,30-acetat to Epidermal Geowth Factor Receptor (1xkk) is -78.9662 (7). Meanwhile, doking score for 12,13-Dihydro-α-amirin-20,30-en-3ol to Epidermal Grwoth Factor Receptor (1XKK) is -74.1941 (lo). Doxorubicin d0cking score is -86.6585 (2). From the results, it can be intered that the two compounds have cytotoxic activities with higher IC50 than the doxorubicin as positive control. In order to obtain more potent cytotoxic activity, the coumpound has to be modifed and tested than using receptor Epidermal Factor receptor.

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Detail Information

Series Title

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Call Number

Majalah Farmasi Indonesia;23(3) 2011: 182-190

Publisher

Yogyakarta : Fakutas FarmasiUGM., 2011

Collation

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Language

English

ISBN/ISSN

0126-1037

Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

plants, medicinal
EUPOTARIUM
TRITERPENES

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