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Spectroscopic Analysis and Cytotoxic Activity of Quas-Sinoid Isolated from the Seeds of Brucea Javanica on Hela Cell

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The quessinoids from Bruce Javanica exhibit bioloical activitas as antimalarial, antitumor, antiviral and antiamoebic activities. These quaasinoids aving a promising antitumor activity. This research was aimed to isolated quassinoid (bruceine A, MW 522 g/mol) compound from B. Javanica and to investigate the cytotoxicity of bruceine A under invitro conditions in Hela cells.The cytotoxicity assays using MTTT asay. The bruceine showed a good cytotoxic activity in Hela cells at 24 hours with doxorubicine as a positive control. Stuctural recored on Tesia av600, (400 MHz) 1HNMR in pyridine (Ds), some in Gyro (300 MHz) 1HNMR i acetone-(d6), DMSO-(06) and CdCI3 13CNMR, 75 MHz, mass spectra on biospectometry LC-MS. Analytical HPLCretention time, ta was 4.617 minutes (Met/H2O) 60;40. Its Molecular formula was determined to be (C26 H34 O11) by the (M+H)+ ion peak at m/z 523 and m/z 1045 confirmed by high Resolution mass spectra Orbitrap (HRESIMS), From the results demonstrated that could be bruceine A compound refers to our spectral data. Its has activity in IC50 value 191,5 µM on Hella under invitro conditions

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Majalah Farmasi Indonesia: 22(2)2011:137-143

Publisher

Yogyakarta : Fakultas Farmasi UGM., 2010

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6p.

Language

English

ISBN/ISSN

0126-1037

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