Analisis Resistansi M. Tuberculosis terhadap Fluoroquinolon dan Penerapan Teknik Biomolekuler Berbasis Nuklir (Analysis of the Resistance of M> Tuberculosis to Fluoroquinolon and the Implementation of nuclear Based Biomolecular Technique) | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Analisis Resistansi M. Tuberculosis terhadap Fluoroquinolon dan Penerapan Teknik Biomolekuler Berbasis Nuklir (Analysis of the Resistance of M> Tuberculosis to Fluoroquinolon and the Implementation of nuclear Based Biomolecular Technique)

Mukh Syifudin - Personal Name; Dewi Deptiani - Personal Name;

Tuberculosis (TB) is still a problem in community health with high rate of mortality. The case became much more complicated due to emerge of Mycobacterium tuberculosis which are reistant to the drugs. This caused the movement of attention from the first line drugs to fluora-quinolon (FQ) as alternative drug. The aim of this research was to do analysis the mutation which causing the resistance of bacterial through nucleic acid alterations wich causing the resistance of bacterial through nucleic acid alternations with polymerase chain reaction (PCR) and single stand conformation polymorphism (SSCP) technique. Analysis was done on gyrA and gyrB genes encoding DNA gyrase of bacterial and closely related to FQ resistance in 100 of sputa samples of positive BTA test results. DNA of M. Tuberculosis strain H3Rv was used as control. From Analysis on gyrA gene it was known that 57 samples were positive PCR and no resistant sample was found. For gyRB gene, only 12 of them were positive PCR and again there was no samples had mutation as cause of resistance. These mean that FQ could be used as replacement drug. Molecular detection technique was know fast and specific for asssessing bacterial resistance. Research poves that reaching for P32-radiosotope labeled DNA alteration was more senitive. Hopefully this results of experiment can be implemented in medication with more effective and support diagnose results so that it will lowering the risk of patiet mortality

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Location name is not set Majalah Farmasi Indonesia; 22(2) 2011:120-127

A0001912

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Series Title: -
Call Number: Majalah Farmasi Indonesia; 22(2) 2011:120-127
Publisher: Yogyakarta : Fakultas Farmasi UGM., 2011
Collation: 7p.
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ISBN/ISSN: 0126-1037
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