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Repellency Test of Wet Tissue Containing DEET (N,N-diethyl-3-metatoluamide) and Citronella Oil (Cymbopogon citratus) Againts Aedes Aegypti Mosquitoes)
Dengue hemorrhagic fever has been one of the major health problems inIndonesia. As the disease spreads out by Aedes aegypti, a variety of wayshas been conducted to disconnect host, agent, and the environment chainincluding prevention of human contact with the vector using by various repellents.The present test compared the complete protection time of N,N-diethyl-3-metatoluamide (DEET) and citronella oil impregnated in wet tissueagainst Aedes aegypti. For this test, an ethanol-based 12.5% DEET and20% citronella oil were prepared, into which dry tissue papers were immersed,drain in open air, and then stored in aluminum foil packs for one dayand one week. Meanwhile, a number of disease-free adult female Aedesaegypti were placed in mosquito cages. The prepared one-day and oneweekstored wet tissues were used to swab volunteer adult human hands(from elbow to wrist) which were then inserted into the mosquito cage inthree replicates (25 mosquitoes each cage). Elapsed time from first hand insertionto the first mosquito bite was calculated and expressed as repellencytime of the impregnated repellent. It was found that the average repellencytimes of one-day stored 12.5% DEET and 20% citronella oil wet tissueswere 4 hour 26 minutes and 14.24 minutes, respectively, while for oneweekstored were 4 hour 6 minutes and 12.57 minutes, respectively. PostHoc test showed that the repellency time difference between one-day andone-week storage was not statistically significance (p = 0.524 for DEET andp = 0.681 for citronella oil).
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