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Pengaruh Suhu dan Interval Waktu Penyimpanan Sampel Serum pada Pengukuran Kadar Glukose Darah

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The number of samples shoudld be chacked often can not be clone immediately examination due to the limited number of laboratory personnel. This phenomenon is common in clinical laboratories and private land. This needs attention given the many factors that can effect test results. Pre-analytical factors that need to be considered is the examination time interval and sample storage temperature prior to examination. Examination of blood glucose is one of the frequent checks at the hospital. The method often used is glucose method witth the serum as a sample. The purpose of this research is to determine how far the impact of delays in the inquest and the influence of storage temperature on serum blood glucose levels. The study was conducted on 30 people consisting of patients and hospial employess who are silling. Criteria for inclusion were men or women aged 20-40 yeras old and willing to follow the research. Exclusion criteria were suffering from diabetes, severe kidney disease, or are pregnant. Each subject and blood samples taken as soon as possible into 3 tubes which will be examined immediate and delayed 4 hours and each tube is stored at a temperature of 2-8⁰c and 25-28⁰c. The parameters examined were the blood chemistry of blood glucose. Examination of blood glucose using glucose oxidase method. Sampling is perforemed by the method of consecutive (consecutive sample). Statistical analysis by idependent samples t-test. The examination of serum samples from 30 patients and hosital employees who meet the criteria of inclusion and exclusion. Comparison of results of blood glucose levels at examination time delay between 0 hourrs and 4 hours to get results that are not significant (p>0.05). and blood glucose levels between groups of temperature 25-28⁰c and 2-8⁰c no significant difference inculcome (p>0.05). The interval 0 hours time and 4 hours at a temperature of 25-28⁰c (room temperature) and 2-8⁰c (refrigerator temperature) does not affect the results of blood glucose levels in serum samples.

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Series Title

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Call Number

Jurnal Kedokteran dan Kesehatan:3(8)2011:39-43

Publisher

Yogyakarta : FK. Universitas Islam Indonesia., 2011

Collation

4p.

Language

ISBN/ISSN

2085-4145

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Edition

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Subject(s)

blood pressure
SERUM
TEMPERATURE
TIME

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