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Laboratory Finding Associated with Positive Blood Culture in Patients Using Intravascular Catheters
The use of intravascular catheter puts patients of risk for catheter-related bloodstream infection (CR-BSI). Laboratory diagnosis of CR-BSI involves blood culture and tip culture. The microorganims detected in positive blood culture and tip culture need to be evaluated whether they are true pathogens of cortaminants. The purposes of this study are to analyse the microbiological profile of positive blood culture and tip culture in patients using intravascular catheter.Furthermore, this study also compare the result of non refrigerated and refrigerated tip culture to infer the value of performing routine tip culture. This uses secondary data on blood culture and tip culture which were collected during the period of January to May 2009 from AusLAB database. Experimental study of refrigerated tip cultures using Maki method was also performated tosupport the analysis on tip culture. Ad data analyzed using descriptive analysis method. In this study, a total of 168 microorganisms were isolated from 150 positive blood culture from 86 patients, The most comon bacteria detected in blood samples were S. Epidemidis (18.45%). To infer CR-BSI among patients, a total 67tips from 65 patients were analysed. Of the 67 tips, 40 tips (60.45%) showed no growth. The concomitant result of blood culture and tip culture is used to assume the probability of CR-BSI among patients,and it was showed that only 3 probable CR-BSI were found. Furthemore, the refrigerated tip culture result showed that most of refrigerated tips (78.05%) yielded the same result as the primary tip culture. Hence refrigeration does not cause significant change. In assisting laboratory diagnosis of CR-BSI, tip culture can be refrigerated until the positive blood culture result is confirmed. Considering the limitations of tip culture, the clinical usefulness of performing routine tip culture need to be evaluated further.
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