Curcumin Analogue (Penatagamavunone-0) Induces Lufeal Cell Apoptosis by Increased Bax/Bcl-2 Protein Ratio | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Curcumin Analogue (Penatagamavunone-0) Induces Lufeal Cell Apoptosis by Increased Bax/Bcl-2 Protein Ratio

Restu Syamsul Hadi - Personal Name; Sri Kadarsih Soejono - Personal Name;

The present study was conducted to investigate the role of curcurnin analogue Pentagamavunone-O (PGV-O) of luteal cell function in apoptosis by examined Bcl-2 and Bax protein expressions. Corpora lutea of rat Sprague-Dawley at 28 days of age after induced by 10 IU pregnant mare's serum gonadotropin (PMSG) were dispersed enzimatically by collagenase. Isolated luteal cells were seeded in 24 well culture plates containing minimal essential medium (MEM) and tetal bovine serum (FBS) until cell had grown to confluence. PGV-O (400 IJM) was immediately treated after they were stimulated by luteinizing honnone (LH), prostaqlandin-Fz, (PGF2a) and LH+PGF2w All these treatments were incubated for 24 hours. Apoptotic luteal cells were analyzed for DNA break by terminal deoxy-nucleotidyl transferase mediated dUTP-biotin nick end la be ling (TUN EL) assay. To examine of ScI-2 and Sax protein expression, sample of plated luteal cells on coverslip were stained by immunocytochemical method. The administration of PGV-O and/or F'GF2u induced luteal cells apoptosis. LH (50 ng/ml) decreases Sax protein expression and inhibits PGV-O-induced luteal cell apoptosis by decresed BaxlBcl-2 protein ratio. Treatment of PGV-O (400 ~M) decreased Scl-2 protein expression, increased Bax protein expression, increased BaxlBcl-2 protein ratio and induced apoptotic luteal cells. It was concluded that PGV-O induces luteal cells apoptosis by increased SaxlBcl-2 protein ratio and decreased apoptotic inhibition by LH.

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Location name is not set Majalah Kesehatan Pharmatika;2(1):110-115

A0001487

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Series Title: -
Call Number: Majalah Kesehatan Pharmedika;2(1)2010:110-115
Publisher: Jakarta : FK Universitas Yarsi., 2010
Collation: 5p.
Language: English
ISBN/ISSN: 2085-5648
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Edition: -
Subject(s): plants, medicinal
curcumin
APOSTOSIS
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