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Expression of p16INK4a Biomarker Has a Diagnostic Value in Predicting the Progressivity of Precancerous Cervical Lesion

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Objective: To evaluate clinical value of p161NK4a biomarker level, by doing p161NK4a immunocytochemistry staining, as a pre¬dictor of progressivity of precancerous cervical lesion.
Method: Design of this research is case-control study which will be stratified. ~search was conducted in Cytology Laboratorium, Gynecology Specialistic Division, Department of Obstetrics and Gynecology, Dr. Cipto Mangunkusumo Hospital from August 2007 to September 2008. Immunocytochemistry examination was con¬ducted in Anatomic Pathology Department, Dr. Hasan Sadikin Hos¬pital. We divided the sample into two categories; patients with and without cervical intraepithelial neo£lasia (CIN). This research will evaluate HPV infection and p16IN 4a biomarker by doing p16INK4a immunocytochemistry (lCC) staining.
Results: We have done immunocytochemistry examination in 130 patients, 26 without CIN and 104 with CIN. Immunocytoche¬mistry cut-off point level is 50, which means value < 50 means low CL) R}6INK4aexpression and in reverse, value ~ 50 means high (H) pl6 K4a expression. The bivariat e analysis of our study were pJ6INK4a(H) expression has the risk ofCIN 1 with OR 8.4, for CIN 2 with OR 13 and for CIN 3 with OR 21, greater than p16INK4a(L) with all p values are significant. ICC p 16INK4a (H) expression, age, number of sexual partners, and education are contributory to the risk of progressivity in CIN. The multivariate analysis demonstrates, ICe expression of pI6INK4a(H) has a risk (OR) of CIN I 17.19 times greater than p 16INK4a (L); p 16INK4a CH) expression's OR for CIN 2 is 25.56; and for OR CIN 3 is 37.32. The expression of p16INK4ahas a significant p value and high OR, thus ICC expression of pl6INK4a is suggested to be included in the algorithm of pre¬cancerous cervical lesion guidelines and scoring of probability of CIN as an alternative to HPV DNA considering less cost of the test. In this research, we found a scoring model to determine probability of progressivity in precancerous cervical lesion.
Conclusion: The expression of p16INK4a has a diagnostic value in predicting the progressivity of precancerous cervical lesion. [Indones J Obstet Gynecol 2010; 34-3: 125-30]

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Series Title

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Call Number

Majalah Obsteric dan Ginekologi Indonesia;34(3)201

Publisher

Jakarta : Indonesia Society of Obstetrics and Gynecology., 2010

Collation

5p.

Language

English

ISBN/ISSN

0303-7924

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Edition

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Subject(s)

CERVICAL INTRACPITHELIAL NEOPLESIA
GENES,PIG

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