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Development of Multiplex-PCR Assay for Rapid Detection of Candida spp.

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Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identifi cation of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specifi city. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp. Methods Five Candida spp. isolates were cultured, identified with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purifi ed by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay. Result DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0,98 pg, 0,98 pg, 0,5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identifi cation of Candida spp.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Medical Journal of Indonesia:19(2)2010:83-87

Penerbit

Jakarta : Faculti of Medicine University of Indonesia., 2010

Deskripsi Fisik

4p.

Bahasa

English

ISBN/ISSN

O853-1773

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

polymerase chain reaction
candidiasis
candida

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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