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Analisis Molekuler Gen NSI Virus Avian Influenza H5N1 yang Diisolasi dari Unggas Asal Purworejo Jawa Tengah dan Bantul Daerah Istimewa Yogyakarta

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Pathogenesis a/infection ofH5NI virus involves hyperinduction a/pro inflammatory cytokines which causes the cytokine storm. Although mu/tigenic, NSJ gene can contribute to the level a/virulence and or cytokine resistance. In this study, the NS1 gene fragment of H5Nl isolated from chicken and muscovy duck in Central Java aljd Yogyakarta, respectively. were analyzed. Phylogenetic analyses were performed in comparison to sequence data of H5Nl viroses from 29 bird isolates and 32 human isolates which were accessed from gene bank in the period of 1997 to 2007. Molecular characterization of amino acid sequences (64-173) revealed a common characteristic of highly pathogenic avian influenza namely, 5 codon deletion (80-84 position). The D92E mutation was not found in these isolates. Phylogenetic analysis demonstrated that the two genetic sequences from AIChickenlPurworejol2005 and AlMuscovyducklBantul12005 have the closest relationship with level of confidence bootstrap value (1000 replicates) as 97.9%. Moreover, the two isolates have closer genetic relationship with isolates from Indonesia than from outside of Indonesia. There is no significant difference between NSI gene fragment sequence motif between birds and human isolates. Three dimensional structure analyses displayed that deletion 80-84 is able to change protein structure ofNSl.

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Series Title

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Call Number

Biomedika; 2(2)2010:79-88

Publisher

Surakarta : FK Universitas Muhammadiyah Surakarta., 2010

Collation

9p.

Language

ISBN/ISSN

2085-8345

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Edition

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Subject(s)

BIRDS
INFLUENZA IN BIRDS
INFLUENZA A VIRUS, H5N1 SUBTYPE

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