Text

Cloning and Molecular Pattern Of HCV 1a NS5a Complete Coding Sequence as Predicting Factor for Resistance Therapy

---

The hepatitis C virus (HCV) NS5A protein is known essential for virus replication and suggested play important role in therapy resistance mechanism. To predict and more understanding the molecular pattern of HCV la NS5A we cloned the NS5A gene ofHCV 1 a isolated in Central of Java Indonesia using standard cloningprocedure. The sequence results were then aligned with all of HCV la NS5A complete coding sequences reported in GenBank. In total, 441 sequences were retrieved and aligned by ClustalW. We revealed the nucleotide and amino acid sequence consensus of HCVNS5Agene. Overall, onlyaa 5, 9-11, 13-15, 18-19,22-23,25-27,29,32-33,35,38-43,45,47,50-51, 55, 57, 59-60, 63,65-67,70,72,76,80,82,84,86,88-91,94-97, lOO, 102,104-106, 109-113, 115-116, 118-120, 125, 128-129, 132, 134,136,140-143,145,147,149-152,154-157,159,162-163, 165, 167-168, 170, 173, 175, 178-179, 184-187, 189- 193,195,201,203,207-208,210,216-218,220, 222-225, 227-235, 238, 243, 249, 252, 257, 268, 270, 271, 274, 278, 283,291-292,298,300,306,309-311,313-314,318-321,324, 326, 329, 331, 336, 338-339, 342, 348, 350, 353, 356, 360,363,368-369,377,405-406,409,411-414,416-419, 421-424, 427, 430-432, and443werefound have no variation. Amino acid variations may have relevant changes of physicochemical properties so that influence the replication efficiency, therefore, the amino acid variations found in the present report need further study.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

Biomedika; 2(22010):47-52

Penerbit

Surakarta : Puslitbang Farmasi, Badan Litbang Kesehatan., 2010

Deskripsi Fisik

5p.

Bahasa

English

ISBN/ISSN

2085-8345

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

HEPATIS C

Info Detail Spesifik

-

Pernyataan Tanggungjawab

-

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar