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Identifikasi Senyawa Antikanker dari Ekstrak Kloroform Kulit Batang Rhizopora Mucronata (The Anticancer Compound Identification From Chlorofrom Extract of Rhizopora Mucronata Stem Bark)

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Previous reasearch reported that chloroform extract that was partitioned from etanol exctracts of R. mucronata stem bark had sitotoxic against Myeloma cells (Diastuti, 2008). This research was aimed to fractinate the chloroform extract of R. mucronata stem bark and cytotoxicity test againts Myeloma cells, then identification the cytotoxic compounds. The ethanol extracts of R. mucronata steam bark was partitioned with chloroform. The chloroform extracts was fractinated by coloum chromatography with gradient eluent were n¬hexane, chloroform, etilacetate and methanol respectivelly. Cytotoxicity test the fraction of chloroform extracts were conducted on Myeloma cells by MTT methods. Identification the compoud of fraction that had a highest cvtotoxic againts Myeloma cells conducted using IR spectrometer, and GCMS. The result showed that 2b fraction of R. mucronata stem bark had a highest cytotoxic againts Myeloma cells, having IC50 equal to 5.122 ~g/mL. Identification of chemical compounds showed that 2b fraction contained two steroid compounds were 4 methyl- colest-24-en-3-01 and 4-methyl-stigmast-22-en-3-01.

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Series Title

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Call Number

Majalah Farmasi Indonesia;21(4)2010:279-284

Publisher

Yogyakarta : Fakultas Farmasi UGM,., 2010

Collation

5p.

Language

ISBN/ISSN

0126-1037

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Edition

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Subject(s)

plants, medicinal
anticarcinogenic agents

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