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Inhibitor Effect of THPGV-0 on the Histamine Release From Antigen-Induced RBL-2H3 Cells (Efek THPGV-0 tehadap Pelepasan Histamin dari Kultur Sel RBL-2H3 dinduksi Antigen)

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Tetrahydropentagamavunon-O (THPGV-O) is assumed to be main metabolite product of biotransformation process of PGV-O. THPGV-O was synthesized by converting PGV-O to the compound by hydrogenation with PdjC as a catalyst. PGV-O potently inhibited the histamine release from ~at mast cells in vitro and in vivo, however, ironically only traces amount of compound was found in the blood. THPGV-O is assumed to have important roles in the biological effects of PGV-O in vivo. In present study, we investigated the antiallergy effect of THPGV-O in compare to this of PGV-O in vitro. The study was performed by using rat basophilic leukemia (RBL-2H3) cell line, a tumor analog of mucosal mast cells. DNP-BSA, an antigen, was used as an inducer for stimulating the histamine release from mast cells. In present study, THPGV-O at low concentration did not succeed to inhibit the histamine release, but at higher concentration (30 and 100 I'M) showed strong effects. THPGV-O at concentration of 100 I'M depleted the histamine release by 96.10 ±O.Sl %. In compare to PGV-O, THPGV-O has higher efficacy but less potent. In the study, the possibilities of the spontaneous release from RBL-2H3 cells by the compounds were also observed. All concentrations of THPGV-O as well as PGV-O showed low spontaneous histamine release, less than 10 % of the total histamine contained in RBL-2H3 cells.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Majalah Farmasi Indonesia;21(4)2010;255-262

Penerbit

Yogyakarta : Fakultas Farmasi UGM., 2010

Deskripsi Fisik

7p.

Bahasa

English

ISBN/ISSN

0126-1037

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

receptors, histamine h1

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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