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Hesperidin Meningkatkan Efek Sitotoksik Doxorubicin pada Sel MCF-7 (Hesperidin Increase Cytotoxic Effect of Doxorubicin in MCF-7 Cells)

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Hesperidin, a flavonoid, shows strong cytotoxic effect in several cancer cell lines. The aim of this research was to investigate cytotoxic activities of hesperidin alone and in combination with doxorubicin. Cell viability assay of hesperidin, doxorubicin, and combination treatments were carried out by using
11 MTT assay. Apoptosis assay was done using double staining method using Ethidium Bromide-Acridine Orange. Hesperidin did not show cytotoxic effect but doxorubicin showed cytotoxic effect with ICso 467 nM. Hesperidin (5, 50 and 100 ~M) increased cytotoxic effect of doxorubicin compared with doxorubicin alone. The strongest cytotoxic activity was showed by the combination of 200 nM doxorubicin and 100 IJM hesperidin. Combination treatment of doxorubicin 200 nM and hesperidin 100 ~M induced apoptosis in MCF-7 cells. Hesperidin is potentially to be developed as co-chemotherapeutic agent for breast cancer, while molecular mechanism need to be explored.

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Detail Information

Series Title

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Call Number

Majalah Farmasi Indonesia 21(1)2010:8-17

Publisher

Yogyakarta : Fakultas Farmasi UGM., 2010

Collation

9p.

Language

ISBN/ISSN

0126-1037

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

CYTOTOXINS
HESPERIDIN

Specific Detail Info

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