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Pengaruh Pemberian Glukosa pada Fyltrat Bakteri Probiotik terhadap Pertumbuhan Salmonela typhi Secara In-Vitro
Probiotics are food additives that are beneficial to health that can improve the balance of microorganisms and protect the digestive tract from pathogenic germs. Type of proven probiotics are beneficial Bifidobacteria and Lactobacillus strains. Prebiotics are oligosaccharides that can not digested by human digestive tract but is able to stimulate the growth of probiotic bacteria.
The study is an experimental research laboratory. The goal is known the effect of sucrose and glucose in the filtrate of probiotic bacteria on the growth of Salmonella typhi in vitro.
The material used is a mixed culture of probiotic bacteria Bifidobacterium bifidum MF 20 / S, Bifidobacterium longum SP 07/3, and Lactobacillus gasseri PA 16/8 which were cultured in MRS Broth media and then be given additional glucose concentration of 0%, 2. S%, S %, 10% and 20%, then the filtrate was taken and the bacteria Salmonella typhi.
The study used diffusion sensitivity test (Kirby-Bauer) and the results were analyzed using non-parametric statistical test with Kruskal- Wellis. Results showed no effect of sucrose and administration of probiotic bacteria glucosa the filtrate with a diameter of inhibition zone formed. The Mann-Whitne!' test concluded that there is a difference of glucose in the filtrate of probiotic bacteria on the growth of Salmonella typhi And note also that the pure filtrate without addition of probiotic addition of sucrose and glucose are not influential in the growth of Salmonella typhi while the addition of sucrose and the addition of glucose ; 2.S%, S%, 10% and
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