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Gene Expression of Manganese Superoxide Dismutase in Human Glioma Cells

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Aim This study analyze the MnSOD gene expression as endogenous antioxidant in human glioma cells compared with leucocyte cells as control.
Methods MnSOD gene expression of20 glioma patients was analyzed by measuring the relative expression ofmRNA and enzyme activity of MnSOD in brain and leucocyte cells. The relative expression of mRNA MnSOD was deter¬mined by using quantitative Real Time RT-PCR and the enzyme activity of MnSOD using biochemical kit assay (xantine oxidase inhibition). Statistic analysis for mRNA and enzyme activity ofMnSOD was performed using Kruskal Wallis test.
Results mRNA of MnSOD in glioma cells of 70 % sample was 0.015-0.627 lower, 10 % was 1.002-1.059 and 20 % was 1.409-6.9 I 5 higher than in leucocyte cells. Also the specific activity ofMnSOD enzyme in glioma cells of80 % sample showed 0,064-0,506 lower and 20 % sample was 1.249-2.718 higher than in leucocyte cells.
Conclusion MnSOD gene expression in human glioma cells are significantly lower than its expression in leucocytes cells. (Med J Indones 2010; 19:21-5)

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Detail Information

Series Title

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Call Number

Medical Journal Indonesia;19(1)2010:21-25

Publisher

Jakarta : Faculty of Medicine University of Indonesia., 2010

Collation

4p.

Language

English

ISBN/ISSN

0853-1773

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

GLIOMA
GENE EXPIESSION
MANGANECE
SUPAROXIDE DISMUTACE

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