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The Effect of Mangan and Lithium on Peripheral Blood Mononucler Cell Viability After Exposure of Narrowband Ultraviolet B in Psoriasis Patients

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Psoriasis is a chronic and recurrent inflammation skin disease characterized with hyperproliferation of keratinocyte mediated by T celllymphocytes. Climatotherapy (bathing and sunbathing) at Dead Sea is model therapy which is effective for moderate and severe psoriasis. However there are group of psoriasis patients who are not responsive to climatotherapy. The success of the model therapy was based on the influence of ultraviolet irradiation which causes depletion of lvmphocvtes. macrophages, and dendritic cells of the skin. Patients who are not respond to climatotherapy shown to have higher level of mangan (Mn) and lithium (U) serum than responsive patients. Mangan and U were suspected influenced the viability of peripheral blood mononuclear cells (PBMC). The aim of the study is to evaluate the effect of Mn and U levels on the viability of PBMC in the serum of psoriasis patient after exposure of narrowband UVB (NB-UVB). The PBMC were isolated from 6 psoriasis patients using Histopaque. Then the cells isolate was incubated in RPMI medium with 0.02 to 0.08pmo1lL Mn and 0.08 to 0.1 pmol/L Li for 3 days. After that the cells were irradiated once with 1/3 to 2 minimal ervthem dose (MED) of NB-UVB. The PBMC viability was measured 3 hours after irradiation with MTT assay and read with ELlSA plate reader. The results showed that increased levels of Mn and Li or combination did not affect on the viability of PBMC at 1/3 to 1 MED UV-UVB. But at higher irradiation dose (2 MED)' the higher level of Mn and U had negative affect in the viability of PBMC after irradiation. It could be concluded that the Mn and Li level in the serum did not affect the viability of PBMC after irradiation of NB-UVB. It was suggest that other cellular component that involved in the development of psoriasis lesions, such as dendritic cells, fibroblasts, macrophages, and keratinocvte. were associated with Mn and Li levels.

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Detail Information

Series Title

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Call Number

Journal of the Medical Sciences:42(1)2010:21-25

Publisher

Yogyakarta : Faculty of Medicine Gadjah Mada Universiti., 2010

Collation

4p.

Language

English

ISBN/ISSN

0126-1312

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Lithium
PSOVIOSIS

Specific Detail Info

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