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Validasi Metode Biooutografi untuk Determinasi Kloramfenikol (Validation of Bioautographi Method for the Determination of Chloramphenicol)

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Contact bioautography method has been developed for determination of chloramphenicol concentration. Validation of bioautography method has beed carried out by parameters including lnearity, accuracy, precision, and detection limit. Thin Layer Chromatography of chloramphenicol has been performed by using Silica gel 60 a stationary phase, chloroform: method (80:20,v/v) and UV lamp as a solvent and for post visualization respectively. Before spotting , analyte of the chloramphenicol was dissolved in aceton as solvent. Bioautography has been performed by using Escherichia coli ATCC 25922 as a bacterial and nutrient Agar as medium test. It was found that one sport visualized on the chromatogram has Rf value 0.5. The result showed that respon of activity to be linear at the amount of chlora,phenicol between 100 ppm 200 ppm, with regression quation: y=2.8x-4.3,r value=0.9 and Vxo=1.8%. Accuracy and predision of the method are 2.8%+2.3 and 96.2%+2.7 respectively. Detection Limit(DL) value is 0.06 µg could be expressed as Minimum inhibition Concen tration (MIC).

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Jurnal Kedokteran Indonesia:1(1):15-24

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Surakarta : FK Universitas Sebelas Maret., 2009

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2086-5171

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