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Effek Ekstrak Etanol Pegagan pada Aktivitas Sekresi Reactive Oxygen Intermedaies (ROI) Makrofag Peritoneal Mencit Swiss Betina Diinduksi Spc-1 (Effec of Ethanolic Extract Herba Oegagan To Activity Of Reactive Oxygen Intermediates Secreation of Peritoneal Macbrophag Spc 1 Induced-male Swiss Mice)

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Macrophage has a very important immunologic role in anticancer defense. Anttivity of macrophages as an anticancer can be assessed by the secretion of reactive oxygen intermediates (ROI). Centella asiatica herb has been used by Indonesian community as enhancing endurance, but its scientific evidence is lacking aimed to 9.2%,23.6%, 22.0± the secretion acvivity of ROI of periotoneal macrophages female swiss induced SPC-t cells after being given a herb Centella asiatic ethanolic extract (EEHP). This study used 21 female swiss mice, weighing between 20-30 grams. Mice are divided into 6 groups. Group 1 as a negative control group has been given peroral aquadest. Group II as a positive control group has been given peroral imboost. Group III, IV, V and VI as the treatment groups, each has been given Centella asiatica athanolice extract at a dose of 1.5,15, 150 and 1500 mg/kgBW/ day peroral for 14 days. On the 15th day, all mice been injected SPC1 cells is subcutaneous area under the breasts and seen the growth of the cancer until the day to-30. After that, on day 31 all mice have been inoculated with SP-C1 cells again. On day 37, all mice have been sacrificed. Perioneal macropahages have been isolated and cultured, the ROI was tested the NBT assay. The data were analyzed using kolmogorov-smimov test and Levene test for normally distributed data and to know homogenelty of variace. Data have been analyzed using Anova test and LSD test with a confidence level of 95%. The result showed the percentage of ROI secretion of each experimental group at doses 1.5,15,150 and 1500 mg/KgBW/day EEHP is 13. 0±2.6%, 22.0± 9.2%, 23.6±3.1%. and 21.6±3.1% and 21.6 ±21.6±6.8%. Doses 15, 150 and 1500 mg/kgBW/day of ethanolic extract of Centella asiatice herb can increase the activity of ROI secretion by peritoneal macrophages of female swiss mice induced SPC-1 cell. Increasing activity of ROI secretion by oeritoneal macrophages of the experimental groups statistically did not differ with the positive control group. Ethanolic axtract of Centella asiatica herb doses 15,150 and 1500 mg/KgBW/day can increase the activity of ROI screation by peritoneal macrophages of female Swiss mice induced SP-C1 cells.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Jurnal Kedokteran Indonesia:1(1):8-14

Penerbit

Surakarta : FK Universitas Sebelas Maret., 2009

Deskripsi Fisik

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Bahasa

ISBN/ISSN

2086-5171

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Tipe Media

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Tipe Pembawa

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Pernyataan Tanggungjawab

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