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Kualitas Spermatozoa dan Aktivitas Enzim Katalse dalam darah Tikus Jantan Galur Sprague Dawley (SD) yang Diradiasi Sinar Ultraviolet (The Quality of Spermatazoa and Activity of Catalytic Enzyme in the Blood of Male Sparague Dawley (SD) Mice Radiated by Ultraviolet Light.)

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The damage of ozone layer in the atmosphere has has reached to a serious extent, due to the increasing ultraviolet (UV) radiation, stemming from the the green house effect and the azone hole. The increasing UV radiation is associated with the risk of some diseases or conditions, e.g. male infertility. The increasing UV ʎʎʎtriggers the existence of reactive oxygen species (ROS), free radicals that contain the oxygen atom-that are deemed to have an important role in male infertility. The excessive concentration of ROS causes oxidative stress as indicated by the activity of catalytic enzyme. This study aimed to examine the quality of spermatozoa and the activity of catalytic enzyme in the blood of male Sprague Dawley (SD) mice radiated by ultraviolet light of ʎ = 200-400 nm. The study subjects were group receiving UV radiation (p1) and a control group (PO). The UV radiation was carried out for 3 hours/day for days. The parameters for spermatozoa included motility percentage, viability, while normality of the spermatozoa morphology was observed by microscope. The activity of catalytic enzyme in the blodd plasma was measured by specroscope. The activity of catalytic in the blodd plasma was measured by spectrophotometry ʎ = 240 nm. The mean of catalytic for Po was 75.2 ± 6.9% and P1: 47.1 ± 4.9%. The mean of spermatozoa motility percentage for was: 64.2 ± 11.3% and P1: 0.7 ±0.7%. The mean of spermatozoa viability percentage for Po was: 72.1 ± 18.3% and P1:28.4± 8.4%. The mean of normal spermatozoa morphology for Po was: 91.3 ± 9.0% and P1:95.2 ± 4.2%. All these differences were statistically significant. Ultra violet radiation impairs the quality of spermatozoa and the activity of catalytic enzyme in the blood of male Sprague Dawley (sd) mice.

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Jurnal Kedokteran Indonesia:1(1):4-7

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Surakarta : FK Universitas Sebelas Maret., 2009

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2086-5171

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