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Perbandingan Efek Ekstrak Eter dengan Ekstrak Etanol Biji Tua Pisang biji Klutuk (Musa balbisiana Colla)pada Sekresi Asam Lambung Tikus Putih in VitroVitro

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Kluthuk banana(musa balbisiana collo), a seeded banana, has been studied for its ability reduce gastric acid secretion. Is the active compound hydrophylic or hydrophobic has not been known yet. The aim of his study is to determine the effect of the seed of ripe kluthuk banana (musa balbisiana colla) etheric and ethanolic extract, and to compare the effect of both extract on rat gastric acid secretion in vitro. This study was conducted using isolated wistar rat stomach, according to the methods modified from Barocelli. Fifty four rats (3-4 month) of 150-250g were used in this study. They were divided into 9 groups (6 rats each). The rats were fasted and drinking water was given adlibitum for 24 hours before testing. The rats were weighted, anesthetized with ether inhalation, and then sacrified . The stomach was taken and suspended in an organ bath containing 37⁰C buffered serosal solution and bubbled with carbogen. The gastric lumen was perfused continuosly with unbuffered mucosal solution 1 ml min-1 and bubbled with 100% o2. The isolated preparation was stabilized for 1 hour and perfusate spilled out. The perfusate was allowed to flow continuously, and colected for 10 minutes duration. The H+ concentration was measured by titration with NaOH 0,001 N using phenolftalein as indicator, known as basal H+consentration. Pretreatment was added to the unbuffered mucosal solution for 30 minutes, after which the treatment (histamine 10-6 M) was added to the unbuffered solution in each group, perfusate gastric lumen were collected every 10 minutes untill 80 minutes and H+ consentration were measured by mean of titration. The H+ consentration elevation was expressed as men ± SEM, the AUCO-80 was calculated and analyzed by ANOVA. The result showed that the total area under curve (AUCO-80) of H+ concentration was 14550 ± 692,70 incontrol group (saline), 141,67 ± 2838,62 in ethetic extract of the seed of ripe kluthuk banana doses+-1 group (EESRKB 1 group) equivalent to 1,92 mg/kgBW, 8516,67 ± 3659/64 in EESRKB II group (equivalent to 3,84 mg/kgBW), 5650±3191,94 in EESRKB III group (equivalent to 7,69 mg/kgBW), 45000 ± 2819,22 in ethanolic extract of the seed of ripe kluthuk banana doses -1 group (AESRKB 1 group) equaivalent to 8,7 mg/kgBW, -5883,33 ± 760,45 in AESRKB II group (equlvalent to 17 A mg/kgBW), -1116,67 ± 3444,36 in AESRKB III group (equavalent to 34,8 mg/kgBW), -1333,33 ± 80,28 in cimetidin group, and 12683,33 ± 968,65 in DMSO group. The statistical analysis showed that the AUCO-80 of EESRKB and AESRKB were significanly (p0,05) than those of AESRKB 1, the AUCO-80 of EESRKB II were sihnificantly higher (P0,05) than those of AESRKB III. It was conduded that the etheric and ethanolic extrac of the seed of tipe kluthuk (Musa balbisiana Colla) showed an inhibitory effect on rat gastric acid secretion induced histamine, and ethanolic extract showed an inhinitory effect more than those of etherix extract.

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Biomedika:1(2):25-29

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Solo : FK Universitas Muhammadiyah Surakarta., 2009

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2085-8345

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