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Efek Antimikroba Minyak Jinten Hita (Nigella Sativa ) terhadap Pertumbuhan Echerichia Coli in Vitro
Black cumin (Nigella sativa) oil contains substances thymoquinone, dithymoquinone, thymohydroquinone, thymol, and tannin which are considered can Inhibit the growth of bacteria, fungi, and parasites, so has an antimicrobial effect. This research is aimed to know the antimicrobial effects. This research is aimed to know the antimicrobial effect of black cumin oil in inhibiting Escherichia coll”s ggrowth. This is a laboratoric experimental research using non random consecutive sampling, with local isolate Escherichia coli aged 24 hours on a nutrient agar and had standardized with Mc Farland 0.5, spread with sterile rib cotton over Mueller-Hinton gell. An empty fiter paper disc as a negative control, 25 µg contrimoxazol antibiotic disc as a positive control, and a filter paper disc filled with black cumin oil, are placed on the Mueller-Hinton gell plate. The plate is incubated for 1x24 hours, then the inhibiting zones is measured. The attempted data were analized with Kruskal-Wallis test. Result of the research shows that in Escherichia coll was formed an inhibiting zone. Kruskal-Walis analysis shows a significant difference (p0,05). The conclusion of the research is black cumin (Nigella sativa) oil has an antimicrobial effect Escherichia coli’s growth.
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