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Increases Interleukin (IL)-4 Expression is Related with Decreases of Bax and Lymphocyte Apoptosis in Bronchiolus and Lung of Asthmatic Mice

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Decrease of lymphocyte apoptosis is one Factor that leads to chronic airway inflammation in allergic asthma.Previous studies revealed the role of interleukin (IL)-4 in preventing lymphocyte apoptosis, however there are few studies about the role of lymphocyte-Bax and its relationship with IL- 4 in asthma. The aim of the study is to demonstrate IL-4 role in preventing lymphocyte apoptosis via lymphocyte-Box inactivation in bronchioli and lungs of asthmatic mice. It is randomized control group experimental study. The subject wasBalb/c mice that categorized into 2 groups: asthma and non-asthma group was sensitzed by ovalbuminintraperitoneally in day 0 and 14, followed by inhalation every 2-3 days for 6 weeks. In week 8, all of mice were termited. The IL-4 and lymphocyte-Bax expression was examined through immunohistochemistry method whereas lymphocyte apoptosis by TUNEL method. Independent sample t-test. Mann Whitney U test, regression analysis, and path analysis was used in statistical analysis with confident interval 95%. The bronchioll and lungs specimene were obtained from 18 mice (9in each group). Lymphocyte apoptosis was similar between 2 groups (p=0.116), additional lymphocyte-Bax decreased in asthmatic group (p=0.003). This indicated low activity lympocyte apoptosis in asthmatic group. Conversely, IL-4 expression increased in asthmatic group (p=0.00). There was moderate negative correlation (r₂ = 0.29, p=0.0110 BETWEEN Il-4 and lymphocyte apoptosis. There was also strong negative correlation (r₂=0.29, p=0.011) between IL-4 and lymphocyte apoptosis. There was also strong negative correlation (r2=o.39, p=0.002) between IL-4 and lymphocyte-Bax expression. However, we did not find any correlation between lymphocyte-Bax and lymphocyte apoptosis (r=0.37, p=0.065). Path analysis revealed the stronger direct relationship between IL-4 and lymphocyte apoptosis rather than indirect relationship via lymphocyte-Bax inactivation. We concluded that increases of IL-4 expressions inhibit lymphocyte apoptosis; however, it is not due to the Bax-lymphocyte.

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Jurnal Kedokteran Brawijaya:25(3):100-110

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Malang : Bagian Ilmu Kesehatan Anak FK UNPAD \r RSHS., 2009

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0216-9347

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