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Profil Kromatogram dan Aktivitas Antibakteri Ekstrak Etano Rimpang Bengle (Zingiber cassumunar Roxb) terhadap Bakteri Ezcherichia Coli In Vitro
Chromato profile and antibacterial activity extract from bengle rhizome (Zingiber cassumunar Roxb). To Echerichia coli in vitro. Bengle (Zingber cassummunar roxb) was a plant which had been used by socien long time aga as a traditional medicine. It was predicted that there were anti-bacteria in bengle, so it might substitutes the utilization of conventional antibiotics. It was predicted that there were anti-bacteria substance in bengla, so it might substitutes the utilization of conventional antibiotics. The aims of this research was to explore the chromatogram and antibacterial activity etanol extract of Bengle Rhizome against Escherichia coli. The method of these research were descriptive and experimental study using the post test only control group design. Ethanol extract of Bengle rhizome was made by maceration method as solvent. Chromatogram profile was using thin loyer chromatography and antibacterial actiwity against escherichia coli was using dilution method and than cultured in Mac Conkey agar media. The results showed thad thin layer chromatography shown 4 spot under UV light 254 nm wave leng with colour, blue violet, blue violet, brown yellow, brown yellow and Rf value 0.21; 0.34, 0.45; o,55. No bacterial growth at etanol of Bengle rhizome 12.5% on Mueller Hinton Browth media, nor at concentration of 25% on Mac: Conkey media. The were 4 compounds predicity included of flovonoid, steroid, volatile oil and terpenoid group. Ethanol extract of bengle rhizone had minimum inhibitory 125% and minimum bactericide concentration 25% against Escherichia coli.
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