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Penentuan LD50 Penyakit Infeksi Toxoplasma Gondii pada Mencit Balb/c

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LD50 determination in toxoplasma gondi infection in balb/c mice , LD50 is a merker of virulence which is reflects degree of microorganism pathogenecity. LD50 of every pathogens to be used should be determined in order to chose an appropiate infective dose of agents under the study. Toxoplasma gondii RH strain has been maintained in the laboratory by serial passage in mice for several years, in which the biologic and laboratory conditions might has an effect an its virulence. The objective of this study was to determine LD50 of Toxoplasma gondii RH strain used for research in Biotechnology Laboratry Gajah Mada University. The design of the study was true experiment consisting three groups of 10 female balb/c mice aged 8-10 weeks that were infected with 10₁,10₃ and 10₆ of Toxoplasma gondii RH strain/mice respectively, and being followed up to day 18. Homogeneity of mice before infection were analyzed by Levence statistic, while effects of infection was analyzed by Anova. The number of ded mice in each group were recorded and LD50 was calculated base on proportional distance. There was no weight difference between group of mice before infection. The weight increased up to day 6 in group 1 and II, while group III after day 3 there was weight decrease. LD50 of Toxoplasma gondii was 1.39x10₃ which belonged to genotype 11 of pathogenicity classification. LD50 of Toxoplasma gondii was 1.39x10₃which is lower than ariginal RH strain, and belonged to group II of genotype, which is often reported as the major canse of human and animal infection.

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Media Medika Indonesiana:43(4):160-165

Publisher

Surabaya : FK Diponegoro dan Ikatan Dokter Indonesia Jateng., 2009

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ISBN/ISSN

0126-1762

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Subject(s)

infection
LETHAL DOSE 50
TOXOPLASMA

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