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Pengaruh PGV-1 dan PGV-2 terhadap Enzim B-Hexosaminidase pada Sel Mast Akibat Induksi Ion Kalsium Intraseluler (The Effect of PGV-1 and PGV on the B-Hex0saminidase release from Intraceller Calcium Ion-Induced Mast Cells)

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PGV-1 or 2,5-bis(4’-hydroxy-3,5’-dimethylbenzylidene) cyclopentanone and RGV-2 or 2,5-bis(4’-hydroxy-3’-5’-diethybenzylidene)cyclopentanone are tro benzylidene cyclopentanone analogues of curcumin. In or study, we ivestiggeted the effects of these compounds on the β-hexoaminidase enzyme relese from mast cell culture (RBL-2H3 cell line,). Thapsigargin and lonomycin were used as intracellular calcium ion stimulants for inducing β-hexoaminidase enzyme relese from mast cells. The release of β-hexoamidase enzymeenzyme was determined by calorimetric methods with substrate, p-nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranocide, and a microplate reader at 405 nm. In present study, treatment of 0.5 µM thapsigargin or 1 µM lonamycin could stimulate the release of β-hexoaminidase enzyme from RBL-2H3 cells by 4.3.91±1.30% dan 52.93±2.07%, respectively, PGV-1 and-2 showed inhibitory effects on the β-hexoaminidase enzyme release from RBL-2H3 cells induced by the increase of intraceluler calcium ion in dose-dependent manner. At the dose of 100 µM, PGV-1 and PGV-2, respectively, inhibited the β-hexoamidase enzyme release by 73.51±8.69% and 66.42±8.63% on thapsigargin experiments, and by 89.73±3,23% and 38.57±5.32% on ionomycin experiments. The IC50 values of their effects on the β-hexoaminidase enzyme release from RBL-2H3 cells, respectively, were 22.20 µM and 22.27 µM on thapsigargin experiment, and 22.77 µM and > 100 µM on lonamycin. Based on the results, the inhibitory effect of PGV-1 and PGV-2 on the β-hexoamidase enzyme release from RBL-2H3 cells involving mechanisms related to the alteration on activation processes of intracellular calcium ion on mast cells.

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Series Title

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Call Number

Majalah Farmasi Indonesia:20(4):207-216

Publisher

Yogyakarta : Fakultas Farmasi UGM., 2009

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ISBN/ISSN

0126-1037

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Subject(s)

calcium
enzymes
MAST CELLS

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