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Morphological Studies of Apoptotic Hela Cella Death Induced by Eurycomanone

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Eurycomanoms is a cytotoxic ingredient found in Eurycoma longifolia jack .Previous studies have noted its activity against many epithellal cell lines. In this study, eurycomanone had abvious cytotoxic effect on Hela cell by methylene blue staining assays. After HeLa were treated with surycomanone, typical morphological changes, including cytoplasm shrinkage and decrease of cell volume were observed by light microscope. Chromatin condensation and nuclear fragmentation could be observed by fluorescence microscops after staining with Hoechst 33258 nucler staining. DNA fragmentation and apoptotic body formation that is characteristic of apoptosis could be determined after treated cells were assayed with TUNEL (terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling). The externalization of phosphatidyl serine (PS) could be determined by flow cytometry analysis using Annexin-v/PI double straining. The result suggested that eurycomanone exerted antiproliferative activity on Hela cells by inducting apoptosis.

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Series Title

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Call Number

Majalah Farmasi Indonesia:20(4):190-197

Publisher

Yogyakarta : Puslitbang Farmasi, Badan Litbang Kesehatan., 2009

Collation

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Language

English

ISBN/ISSN

0126-1037

Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

CYTOTOXINS
HELA CELLA
EURYCOMA
APOPTOTOXINS

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