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Evaluasi Waktu Mati Kromatogrefi untuk Penentuan Indeks Retensi pada RP-HPLC Menggunakan Beberapa deret Homolog (Evaluation of Chromatographic deed times for Retention Indices Determination in Rp_HPLC Using Some Homologous Series)

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Dead time that defined as a retention time of unretained substance in the chromatographic coloum, is needed to determine all retention parameters in cloum chromatography such as corrected retention time, relative retention time and kovats Retention Indices. This research reported a comparison between the iteration and lenearization of corrected retention times of homologous series such as n—alkane, akylryketone, alkybenzene and 2-alkanone. Furthermore the iteration method provides better dead-time values and smaller standard deviations than the lenearization method. Moreover, the dead-time calculation obtained according to homologous series is not depending on solvent composition for verious homologous series. The n-alkane homologous series show better indication accuracy of fit (S/N) in comparison with other homologous series, thereafter 2-alakanone exhibit the second best adjustment.

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Series Title

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Call Number

Majalah Farmasi Indonesia:20(3):133-140

Publisher

Yogyakarta : Fakultas Farmasi UGM., 2009

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ISBN/ISSN

0126-1037

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Edition

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Subject(s)

drug therapy
CHORMATOGRAPHY, HIGH PRESSURELIQUID

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