Fraksi n-Butanolik Kapang Endofit Buah Makasar Meningkatkan Efek Apoptosis Doxorubusin pada Sel MCF-7 (n-Butanolic Fraction of Endofic Fungi of Buah Makasar Increases Apoptotic Effect of Doxorubicin on MCF-7 Cells) | Perpustakaan Badan Kebijakan Pembangunan Kesehatan

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Fraksi n-Butanolik Kapang Endofit Buah Makasar Meningkatkan Efek Apoptosis Doxorubusin pada Sel MCF-7 (n-Butanolic Fraction of Endofic Fungi of Buah Makasar Increases Apoptotic Effect of Doxorubicin on MCF-7 Cells)

Shirly Kumala - Personal Name; Edy Melyanto - Personal Name; Endah Pudji Septisetyani - Personal Name;

Makassar fruit, Brucea Javanica (L) Merr., showed chemopreventive activity. Secondary metabolites come from B. Javanica fruit, brucatol g/mL dan 148 nM, respectively. Both of BF and doxorubicin showed apoptosis induction on IC50. Combination of BF-doxoand bruceantine, induced cell differentiation and apoptosis on Leukemia cell, while quessioid and its derivates acted as antitumor promoter. Butanolic farction of supernatan of endofitic fungi 1.2.11 isolate fermentation which isolated from B. Javanice fruit showed cytotoxycity toward sveral cancer cells. This fraction has been identified as Bruceosin and Canthin-6-one derivates. Butanolic fraction (FB) of supernatan from endofitic fungi 1.3.11 isolate fermentation is predicted for having similar cytitoxycity as active as 1.2.11 isolate. This research is aimed to exploere cytotoxycity potention dan apoptosis induction of BF from 1.3.11 isolateand combination effect of BF-doxorubicin on MCF-7 breast cancer cell. Synergism of BF-doxorubicin combination detect from cell viabilityinhibition and apoptosis induction on MCF-7, a breast cancer cell lines which shows resistancy toward doxorubicin. Cell viability on single treatment of BF and doxorubicin and its combination were carried out by MTT assay to determine IC50 and combination index (CI). Apoptosis induction bromideacridine orange DNA staining. N-Butanolic fraction and doxorubicin showed cell viability inhibition on MCF-7 cel with IC50 48 µg/mL dan 148 nM, respectively. Both of Bf and doxorubicin showed apoptosis induction on IC50. Combination of FB-doxorubicin showed synergism and increased apoptosis induction on MCF-7 cell.

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Location name is not set Majalah Farmasi Indonesia:20(1):42-47

A00002960

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Series Title: -
Call Number: Majalah Farmasi Indonesia:20(1):42-47
Publisher: Yogyakarta : Fakultas Farmasi UGM., 2009
Collation: -
Language
ISBN/ISSN: 0126-1037
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Content Type: -
Media Type: -
Carrier Type: -
Edition: -
Subject(s): PLANT, MEDICINAL
APOPTOSIS
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