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Peranan Isozim Protein Kinase C-Alpha dalam Memediasi Efek Antiproliferasi Alpha-Tokoferol pada Sel Stroma Prostat

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Alpha tocopherol, also has a non anti-oxidant activity that corrrelates with inhibition of PKC-alpha isozyme activity. Several studies confirmed its role in ihhibition of vascular smooth umscle proliferation. Inhibition of stromal cell proliferation is an alternative method of prevention of BPH. The aim of this in vitro study is to confirm that alphatocopherol, by its ability to inhibit PKC-alpha activity, has the capability to inhibit human prostatic stromal cell proliferation. Primary cultures of human prostatic stromal muscle cells were obtained from 4 patients with blader outlet obstruction. The effects of various concentration of alpha-tocopherol (20,30,40,50 and 60 microM) were examined by cell proliferation and PKC-alpha isozyme actiity assay. The cell proliferation studywas performed using the 3-(4,5-dimenthylthizol-2 yl)-2,5-diphenyterazolium bromide (MTT) assay method. A caspase-3 activity assay was also performed to examine apoptotic mechanism. The MTT method, which is used to count viable cells, showed that exposures to various doses of alpha-tocopherol resulted in decresed proliferation rate. The decrement of proliferation rate is linear with the increment of alpha-tocopherol dose. Immunocytochemistry staining with anti caspase-3 antibody showed that no cells developed apoptosis. Alphatocopherol also inhibits PKC-alpha activity. Isozyme activity is reduced along with the increment of the dose (Dosedependent). There is a correlation between PKC-alpha activity with cell proliferation rate after exposure to alphstocopherol). There is a correlation between PKC-alpha activity with cell proliferation rate after exposure to alphatocopherol (R2=0,9279; p=0,000). Alpha-tocopherol at different concentrations reduces prostatic stromal cell proliferation, without an effect on apoptosis. This antiperoliferation property correlates with a decreased activity of PKC-alpha isozyme.

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Series Title

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Call Number

Jurnal Urologi Indonesia: 16(2): 29-36

Publisher

Surabaya : Ikatan Ahli Urologi Indonesia., 2009

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ISBN/ISSN

0853-4438

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Edition

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Subject(s)

ISOENZYMES
STROMAL CELLS
PROTEIN KINASE C-ALPHA

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