Text

The Development of Allergic Mouse Model Following Chronic Ovalbumin Exposure

---

Histology expression in asthma show inflammatory processes and airway structural changes. Treatment development in asthma needs appropriate animal models but most animal models use short term allergen exposure. The aim of this research was to create an allergic mouse model with allergic inflammation and structureal changes histology expression. Eight female BALB/c mice, 6-12 weeks old, 80-100 grams were devided into groups. The first group were exposed to a normal saline solution where the second group received ovalbumin. Mice were sensitilized by intraperitoneal injection of avaibumin on Day 0 and 14 and chellenged by nebulization of 1% ovaibumin three weekly for 6 weeks. Twenty fours after final exposure, the mice were sacrificed by lethal cardiac puncture. The level of total IgE in serum were measured by EISA and IL-4 receptor expression were examinated by immunohistochemistry. Morphometric analysis was performed to count inflammatory cels, eosinophil, goblet cells, epithel and smooth muscle thickness. Differences betwen the control and exposed mice were analyzed by Mann-Whitney test. There were a significant differences in total IgE serum expression IL-4 receptor between the two groups (P

---

Availability

Detail Information

Series Title

-

Call Number

Jurnal Kedokteran Brawijaya:25(1): 1-5

Publisher

Malang : Fakultas Kedokteran Universitas Brawijaya., 2009

Collation

5p.

Language

ISBN/ISSN

0216-9347

Classification

-

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

asthma
RATS-diseases
OVALBUMIN
HYPERTENTIVITY

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment