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Profil dan Identifikasi Serum Amiloid Protein (SAP) sebagai Protein Fase Akut pada Mencit yang Dipapar Salmonella Enteritidis (Profile and Indentify the Serum of Amiloid Protein (SAP) as Acute Phase Protein in at Mice Which Inoculated Salmonela Enteritidis)

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This research have a purpose to identify SAP as acute phase protein from mice which inoculated Salmonella enteritis with the method SDS. PAGE. Its important is role SAP in detecting early case of infection and inflammation, as protector of infectif agent and prevent the culture degradasi pass role antiproteinase, so that require to be isolation SAP. Used 80 mouse old three months of mean weight 25 g, decided our treatment group each treatment group consisted five mouse inokulasi with the bacterium of Salmonella enteritidis 10⁶, 10⁸, 10⁹ and 10¹⁰ cell / ml by intraperitoneal. Later at 20, 32, 38 and 44 hours after inoculated by removed blood serum, protein level rate frotein level from serum detected by chromotografi. Serum which have been collected is ready to weared to be identified with the Sodium pf Dedecyl Sulfate Polyarylamide of Gel Electroforesis (SDS Page) to Know the to molecular weight. Result have been identified by the serum protein from mouse of molecular weight equal to 25,89 kDa that suggest SAP. The Protein identified from mouse which infection of salmonella enteritidis of equal to 10⁶ and 10⁸ at collection 20 and 44 hours afterinoculation.

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Series Title

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Call Number

Jurnal Penelitian Medika Eksakta:8 (1): 1-7

Publisher

Surabaya : Lemlit dan Pengamdian Unair., 2009

Collation

7p.

Language

ISBN/ISSN

1411-6626

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Edition

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Subject(s)

Salmonella enteritidis
SERUM ANMYLOID a PROTEIN

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