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Interaction between Stelat Cell and Marcofag on Liver Abscess of Strain Balb/c Mice Inducted with Tetrachloride Carbon (Interaksi antara Sel Stelat dan Makrofag pada Proses Peradangan Hati Mencit Galur Balb/c yang Diinduksi dengan Karbon Tetraklorida)

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To observe interaction between stellate cells and macrophages/Kupffer cells in the liver tissue with cellular and molecular changes during the process of inflammation, sixteen male mice of Balb/c strain weighing 25-30 g were used as control group and three treated group that received single subcutaneous injection of CCl4 0.3 ml/kg BW and killed in day 3.5 and 7 after injection respectively. With immunocytochemistry staining method, anti-desmin antibody and anti-MOMA 2 antibody were applied for identification of stellate cells (Ito cells, Fat-storing cells) and macrophages/Kupffer cells. For ultrastructure description of liver cells, the liver tissue were blocked in Poly/bed 812 and observed with transmission electron microscopy method. In treated group of day 3, necrotic parenchymal cells were found surrounding central veins accompanied with inflammatory cells. After five days, clear foci consisted of stellate cells, macrophages and other inflammatory cells were seen in the degenerating area. Those foci developed and occupied with those cells and cells to form granuloma. An intimate contact between stellate cells and macrophages/Kupffer cells within the granulomas suggested the increasing number of those cells significantly (p less than 0.001). Abundant collagen fibers were found in the side of stellate cells that identified with small lipid droplets and well developed rER. It is suggested that in the process of hepatic inflammation intoxicated with CCl4, granuloma formation as a well-organized microenvironment in the liver tissues provided a suitable domain for interaction between stellate cells and macrophages.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

Majalah Kedokteran Bandung,27(4)1995:131-135

Penerbit

: ., 1995

Deskripsi Fisik

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Bahasa

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

liver abscess

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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